SCC293
U2OS EGFP-H2B Human Osteosarcoma Cell Line
Human
Synonym(s):
U2OS EGFP-H2B Human Osteosarcoma Cell Line
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
product name
U2OS EGFP-H2B Human Osteosarcoma Cell Line,
biological source
human
packaging
pkg of >1X10⁶ vials
manufacturer/tradename
Sigma-Aldrich
growth mode
N/A
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
≤ − 140°C
General description
Chromosomal abnormalities underlie a substantial proportion of cancers, including most solid tumors (1). Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrange
Application
Chromosomal abnormalities underlie a substantial proportion of cancers, including the majority of solid tumors.1 Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrangements.1 Understanding the mechanisms of chromosomal instability is key to unlocking the multifaceted roles that they play in cancer etiology and progression. The U2OS osteosarcoma is one of the first and most widely used cancer cell lines, especially for the study of mitosis, apoptosis, and the cellular mechanisms that give rise to cancer. U2OS cells undergo chromosome breakage and chromothripsis at high frequency.2 The expression of EGFP fused to a histone marker in the U2OS EGFP-H2B cell line allows for visualization of chromosome dynamics by light microscopy, a powerful tool in unraveling the complex interactions and molecular players involved in chromosomal instability.3 Expression of EGFP-H2B in this cell line is maintained via selection with blasticidin. The U2OS EGFP-H2B cell line facilitates expanded insights to chromosomal biology, allowing immediate relevancy to the many studies using the well-characterized U2OS background.Source The U2OS EGFP-H2B Human Osteosarcoma cell line was derived from U2OS cells stably transfected with a construct expressing EGFP-histone-H2B.3 The parental U2OS cell line was derived from an osteosarcoma from a 15-year-old female.2References1. Thompson SL, Compton DA. Chormosome Res. 2011; 19(3):433-444.2. Pontén J, Saksela E. Int J Cancer. 1967; 2(5):434-447.3. Wang H et al. Oncotarget 2017; 8(30):48671-48687.
Each vial contains ≥ 1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.
Features and Benefits
U2OS EGFP-H2B Human Osteosarcoma Cell Line allows for visualization of chromosome dynamics by light microscopy
Storage and Stability
The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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