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Anti-HUNK Antibody

Name: Anti-HUNK Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

NCBI accession no.

NP_055401

UniProt accession no.

P57058

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HUNK(30811)

Description

General description

Hormonally up-regulated neu tumor-associated kinase (HUNK) belongs to the protein kinase superfamily and is an SNF1-related serine/threonine kinase that has a unique COOH-terminal (C-terminal) part without homology to any known protein. This protein is highly expressed in the mammary gland, kidney, and central nervous system.

~70 kDa observed. The calculated molecular weight is 80 kDa, however HUNK has been shown as a ~70 kDa band in western blots. (Gardner, H.P., et al. (2000). Development. 127:4493-4509.)

Immunogen

GST-tagged recombinant protein corresponding to human HUNK.

Application

Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected HUNK in human brain and human cerebellum tissues.

This Anti-HUNK Antibody is validated for use in WB, IH(P) for the detection of HUNK.

Biochem/physiol Actions

Other homologies: Mouse (87% sequence homology) and Rat (85% sequence homology).

Analysis Note

Control
SK-BR3 cell lysate

Evaluated by Western Blot in SK-BR3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected HUNK on 10 µg of SK-BR3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Show Differences

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This Item	SAB2900162	ABC213	SAB4500475
Sigma-Aldrich ABC91 Anti-HUNK Antibody	Sigma-Aldrich SAB2900162 Anti-HUNK antibody produced in rabbit	Sigma-Aldrich ABC213 Anti-phospho ULK1 Antibody (Ser777)	Sigma-Aldrich SAB4500475 Anti-NEK7 antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
Quality Level 100	Quality Level -	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable	technique(s) inhibition assay: suitable (peptide), western blot: suitable	technique(s) ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000
UniProt accession no. P57058	UniProt accession no. P57058	UniProt accession no. O70405	UniProt accession no. Q8TDX7

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c