分子生物學用四鹽酸精胺

Product No. S1141

CAS RN 306-67-2
EC 206-189-8
異名：N, N'-Bis(3-aminopropyl)-1,4-butanediamine tetrahydrochloride

產品說明

本產品適用於從低鹽水性緩衝液中沉澱 DNA。

精胺是一種天然多胺，存在於所有真核生物中，但在原核生物中較少見。²精胺是由精胺合成酶在精胺上加上氨基丙基而形成的。精胺具有強鹼性，在生理 pH 值的水溶液中，它的所有胺基都會帶正電。³ 已經有文獻評論精胺和其他多胺在影響 RNA 結構和蛋白質功能上的作用。⁴

精氨酸常用於分子生物學和生物化學研究。精胺在溶液中的多陽離子特性使其可用於從低鹽水性緩衝液中沉澱長度為 100 個碱基對的 DNA，以及從脈衝場凝膠中分離 DNA。^5,6,7精胺也可用於染色體的分離和染色質的聚集。^10,11研究了精胺與 DNA 的複合作用，以形成直徑為 100 nm 的微粒。^13,14

據報導，精胺能與 N-methyl D-aspartate receptor complex 相關的甘氨酸結合位點互動。¹⁵精胺已被證實可抑制大鼠小腦和培養的小腦粒神經元細胞製劑中神經元一氧化氮合酶將 L-精氨酸轉換為 L-瓜氨酸。¹⁶ 在急性海馬切片中，1 mM精胺透過阻斷 NMDA 受體和電壓活化的 Ca²⁺ 通道而發揮神經保護作用。¹⁷ 研究了精胺對 MK-801 與重組 N-methyl-D-aspartate 受體結合的影響。¹⁸蛋白質和蛋白質複合物已經用精胺結晶。^18,19精胺的其他應用包括在 MALDI-MS 中作為基質，用於分析糖共聚物和寡核苷酸。^19,20

注意事項與免責聲明

本產品僅供 R&D 使用，不得用於藥物、家用或其他用途。請參閱安全資料表，以瞭解有關危害和安全處理實務的資訊。

製備說明

本產品可溶解於水（100 mg/mL），生成無色至淡黃色的透明溶液。

儲存/穩定性

精胺游離基的溶液很容易被氧化。

如果在脫氣水中製備溶液，並在氬氣或氮氣下以冷凍等分的方式儲存，溶液最為穩定。

產品簡介

分子式：C₁₀H₂₆N₄ - 4HCl
分子量：348.18
熔點：312 - 314.5 °C²¹

參考資料

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13. Hu J, Mahmoud MI, El-Fakahany EE. 1994. Polyamines inhibit nitric oxide synthase in rat cerebellum. Neuroscience Letters. 175(1-2):41-45. https://doi.org/10.1016/0304-3940(94)91073-1

14. Ferchmin P, Pérez D, Biello M. 2000. Spermine is neuroprotective against anoxia and N-methyl-d-aspartate in hippocampal slices. Brain Research. 859(2):273-279. https://doi.org/10.1016/s0006-8993(00)01973-9

15. Sharma TA, Reynolds IJ. Characterization of the effects of polyamines on [125I]MK-801 binding to recombinant N-methyl D-aspartate receptors. J. Pharmacol. Exp. Ther., 289(2), 1041-1047 (1999)..

16. Xie X, Rao S, Walian P, Hatch V, Phillips GN, Cohen C. 1994. Coiled-coil packing in spermine-induced tropomyosin crystals a comparative study of three forms. Journal of Molecular Biology. 236(4):1212-1226. https://doi.org/10.1016/0022-2836(94)90022-1

17. Qian C, Lagacé L, Massariol M, Chabot C, Yoakim C, Déziel R, Tong L. 2000. A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex. Acta Crystallogr D Biol Cryst. 56(2):175-180. https://doi.org/10.1107/s0907444999015103

18. Mechref Y, Novotny MV. 1998. Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix. J Am Soc Mass Spectrom. 9(12):1293-1302. https://doi.org/10.1016/s1044-0305(98)00106-8

19. Asara JM, Allison J. 1999. Enhanced Detection of Oligonucleotides in UV MALDI MS Using the Tetraamine Spermine as a Matrix Additive. Anal. Chem.. 71(14):2866-2870. https://doi.org/10.1021/ac981406l

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