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Merck

TLC Analysys of Antioxidative Flavonoids in Plant Extracts on Silica Gel 60 F254s

TLC Analysys of Antioxidative Flavonoids in Plant Extracts on Silica Gel 60 F254s application for TLC

材料

附屬品

產品號碼
描述
訂價

瓶形喷雾器

capacity 240 mL

矩形 TLC 展开罐,完整

L × H × W 27.0 cm × 26.5 cm × 7.0 cm

Sigma 薄层色谱喷雾盒

Spectroline®Spectroline E 系列紫外灯

output8 W, AC/DC input 115 V AC

矩形 TLC 展开罐,完整

L × H × W 7.5 cm × 15.5 cm × 8.0 cm

Hamilton® 薄层色谱注射器

1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),

coated tip

Spectroline®Spectroline E 系列紫外灯

output6 W, AC/DC input 120 V AC

Hamilton® 薄层色谱注射器

1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Hamilton® 薄层色谱注射器

1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),

coated tip

矩形 TLC 展开罐,完整

L × H × W 17.5 cm × 16.0 cm × 8.2 cm

Spectroline®Spectroline E 系列紫外灯

output6 W, AC/DC input 230 V AC

Spectroline®Spectroline E 系列紫外灯

output4 W, AC/DC input 230 V AC

Spectroline®Spectroline E 系列紫外灯

output8 W, AC/DC input 230 V AC

Latch-lid 薄层色谱展开槽

For use with 10 cm × 10 cm plates

Hamilton® 薄层色谱注射器

1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Latch-lid 薄层色谱展开槽

For use with 20 cm × 20 cm plates

Spectroline®Spectroline E 系列紫外灯

output4 W, AC/DC input 115 V AC

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

capacity 20 μL, accuracy: ±0.5%

用于薄层层析板的试剂喷雾器

volume 250 mL, Bottle-Type

CONDITIONS

sample preparation

Extract samples were filtered with a 0.45 μm syringe filter. Application by CAMAG ATS4 6mm bandwise.

plate

1.15445 HPTLC LiChrospher Silica gel 60 F254s 20 cm x 10 cm (1.15445.0001)

eluent

[A] ethylacetate; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C:D) containing 0.1% n-Heptane

migration distance

5 cm

chamber

normal chamber without chamber saturation

detector

366 nm Diphenylboric acid-2-aminoethylester-reagent for Flavonoids, Vanillin-sulfuric acidreagent e.g. for Phenols, Steroids, Glycoside; DPPH reagent for antioxidants (visible light)