TLC Analysis Of Vitamin C Derivates From Cosmetic Formulation on HPTLC Silica gel 60 F254s

sample preparation

1 g cosmetic formulation (1% and 2% active) was stirred in 10 ml THF for 15 min and filtered over a 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.

plate

1.15161 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151161.0001)

eluent

[A] methanol; [B] acetonitrile; (9:1, A:B)

migration distance

6 cm

chamber

normal chamber without chamber saturation

sample

0.150 μL

detector

UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.)