TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF254s

材料
附屬品
產品號碼
描述
訂價
Hamilton® 薄层色谱注射器
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 wF 254s with concentrating zone (1.13124.0001)
eluent
[A] methanol; [B] acetonitrile; [C] water; (30:70:5, A:B:C)
migration distance
6 cm
chamber
normal chamber without chamber saturation
sample
5 μL
Dipped in MnCl2-sulfuric acid heated up to 110 C for 10 min in situ evaluation (with TLC/HPTLC scanner (Camag) UV 366 nm)