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Merck

LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18

LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18 application for LC-MS

材料

分析柱

產品號碼
描述
訂價

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 0.1% (v/v) formic acid in water; [B] 0.1% (v/v) formic acid in 75:25 (v/v) acetonitrile:water

flow rate

0.2 mL/min

gradient

2% B for 2 min, to 100% B in 38 min

column temp.

35 °C

detector

ESI(+) TOF, extracted ions m/z 463.1244, 433.1140, 505.137

injection

1 μL

描述

分析報告

The purpose of this study was to evaluate the change in anthocyanins levels in wild blueberries as a function of berry development. The delphinidin, putinidin, peonidin, and malvinidin base-modified components exhibited a dramatic increase in abundance as a function of berry development. The cyanidin base-modified components  exhibited a much lower increase in abundance as a function of berry development, it is uncertain the significance of the rate increase. Chlorogenic acid exhibited a continuous decrease in abundance as a function of berry development. Overall, the combination of UV/Vis detection along with accurate mass TOF enabled a effective method of identification of anthocyanins from blueberry extracts. The Ascentis Express column provided rapid, sensitive detection and rugged operation.

其他說明

Berries (1.0 g) were added to 1.0 mL of 1% (v/v) formic acid in methanol. Samples were crushed in the solvent mixture and extracted (refrigerated) for 2 hours. A portion of the extracted sample was removed, centrifuged and the supernatant was collected for HPLC analysis.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany