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Merck

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18 application for HPLC

材料

分析柱

產品號碼
描述
訂價

581324-U

Ascentis ® C18 HPLC 色谱柱

5 μm particle size, L × I.D. 15 cm × 4.6 mm

標準

產品號碼
描述
訂價

47763

胆钙化固醇（维生素 D3）

analytical standard

47768

麦角钙化甾醇 (D2)

analytical standard

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

acetonitrile

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 290 nm

sample

50 μg/mL each in acetonitrile

描述

分析報告

This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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