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HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate application for HPLC

材料

分析柱

產品號碼
描述
訂價

Z777033

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

標準品

產品號碼
描述
訂價

M1882

肌红蛋白 来源于马心脏

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

U0750

尿嘧啶

≥99.0%

T1001

甲状腺球蛋白 来源于牛甲状腺

powder, ≥90% (agarose gel electrophoresis)

I4131

IgG 来源于大鼠血清

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

05470

牛血清白蛋白 来源于牛血清

lyophilized powder, crystallized, ≥98.0% (GE)

流動相成分

產品號碼
描述
訂價

452289

磷酸

≥85 wt. % in H2O, ≥99.999% trace metals basis

CONDITIONS

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm (Z777033)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid

flow rate

0.1 mL/min

pressure

173 psi (12 bar)

column temp.

25 °C

detector

UV, 215 nm

injection

4 μL

sample

protein standards

描述

分析報告

Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.

法律資訊

Zenix is a registered trademark of Sepax Technologies

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS