HPLC analysis of Methyl Paraben and Organic Acids on Ascentis® Express RP-Amide
CONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (50774-U)
mobile phase
[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)
flow rate
1.3 mL/min
pressure
1870 psi (129 bar)
column temp.
25 °C
detector
UV, 254 nm
injection
5 μL
sample
[A] water; [B] acetonitrile; (50:50, A:B)
描述
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany