HPLC Analysis of Cetirizine Hydrochloride on Purospher^® STAR RP-18 endcapped

column

Purospher^® STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)

mobile phase

[A] 100mM NaH₂PO₄ aqueous solution, adjusted to pH 3.7 with H₃PO₄; [B] acetonitrile; (60:40, A:B)

flow rate

1 mL/min

pressure

1465 psi 101 Bar (101 Bar)

column temp.

30 °C

detector

232 nm cell: 1 μL/10 mm

injection

20 μL

sample

Weigh appropriate amount of Cetirizine, add diluent to obtain 100 μg/mL (100 ppm) concentration.