HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery^® RP-Amide C16

column

Discovery RP-AmideC16, 25 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

[A] 0.1% TFA, 1% acetonitrile in water; [B] 0.1% TFA, 40% acetonitrile

gradient

0-100% B in 130 min

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 215 nm