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Merck

HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm

HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm application for HPLC

材料

分析柱

產品號碼
描述
訂價

50537-U

Ascentis® Express C18，5μm HPLC色谱柱

5 μm particle size, L × I.D. 15 cm × 4.6 mm

標準

產品號碼
描述
訂價

75199

阿齐霉素

流動相成分

產品號碼
描述
訂價

452289

磷酸

≥85 wt. % in H2O, ≥99.999% trace metals basis

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 μm particles (50537-U)

mobile phase

[A] 10 mM potassium phosphate, dibasic, pH 7.0 with phosphoric   acid; [B] methanol; (20:80, A:B)

flow rate

1.5 mL/min

pressure

2558 psi (176 bar)

column temp.

45 °C

detector

UV, 210 nm

injection

10 μL

描述

分析報告

A simple high-performance liquid chromatography (HPLC) method was developed for the determination of azithromycin in raw materials and pharmaceutical formulations without any pretreatment or derivatization step.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS