HPLC Analysis of Asparaginase on Zenix^® SEC-150

column

Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles (Z777018)

mobile phase

150 mM sodium phosphate, pH 7.0

flow rate

0.6 mL/min

pressure

1015 psi (70 bar)

column temp.

ambient

detector

UV, 280 nm

inj. temp.

5 μL

sample

asparaginase (2.0 mg/mL in water), RT 10.895 minute, MW 5000-6000, pI 4.85