GC Analysis of PUFA No. 3 Mix (Menhaden Oil Polyunsaturated Fatty Acid (PUFA) Methyl Esters) on Omegawax (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

column

Omegawax 100, 15 m × 0.10 mm I.D., 0.10 μm (23399-U)

oven

140 °C, 40 °C/min to 280 °C (2 min)

inj. temp.

250 °C

detector

FID, 280 °C

carrier gas

hydrogen, 50 cm/sec constant

injection

0.2 μL, 200:1 split

liner

4 mm I.D., split, cup design

sample

PUFA No. III – Menhaden Oil (47085-U), diluted to 50 mg/mL in methylene chloride