ZRB2179

Anti-BTG2 Antibody, clone 1G17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Protein BTG2;BTG family member 2;NGF-inducible anti-proliferative protein PC3
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1G17, recombinant monoclonal
• 描述: 1G17 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 17.42 kDa; observed mol wt ~18 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: Internal
• Protein ID登錄號: NP_006754
• UniProt登錄號: P78543
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... BTG2(7832)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1G17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects BTG2. It targets an epitope within 21 amino acids from the internal region.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human BTG2.

應用

Quality Control Testing

Evaluated by Western Blotting in Human Cerebellum tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BTG2 in Human Cerebellum tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BTG2 in Human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BTG2 in human cerebellum tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound BTG2 with a KD of 5.8 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Protein BTG2 (UniProt: P78543; also known as BTG family member 2, NGF-inducible anti-proliferative protein PC3) is encoded by the BTG2 (also known as PC3) gene (Gene ID: 7832) in human. BTG2 serves as an anti-proliferative protein whose expression is induced through a p53-dependent mechanism and its levels are significantly elevated in response to DNA damage. It plays a role in the negative control of cell proliferation and transfections of a BTG2 mammalian expression vector into NIH3T3 cells is reported to cause a decrease in their growth rate and cloning ability. BTG2 acts as a tumor suppressor and its levels are down-regulated in many human cancers. The low expression of BTG2 is found in many human cancer tissues and its loss is related to cell migration and invasion. BTG2 is reported to inhibit the proliferation and invasion of gastric cancer cells. Its overexpression can suppress the cell growth and promote apoptosis in pancreatic cancer cells. Its overexpression also reduces the levels of cyclin D1, MMP-1, and MMP-2, and suppresses the proliferation of lung cancer cells. BTG2 is one of the PRMT1 binding partner and its binding increases PRMT1 activity, which is essential for neurite outgrowth. BTG2 is phosphorylated at serine 147 by ERK1 and ERK2 and at serine 149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mao, B., et al. (2015). Int. J. Oncol. 46(2):459-464; Miyata, S., et al. (2008). Neurosci. Lett. 445(2); 162-165; Rouault, J-P., et al. (1996). Nat.Genet.14(4); 482-486).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1