ZRB1407

Anti-Anillin Antibody, clone 2C6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: ANLN, Anillin actin binding protein
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 124.2 kDa; observed mol wt ~130 kDa
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: Q9NQW6
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ANLN(54443)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 2C6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Anillin. It targets an epitope within the N-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal region of human Anillin.

應用

Anti-Anillin, clone 2C6 ZooMAb, Cat. No. ZRB1407, is a recombinant Rabbit monoclonal antibody that specifically targets Anillin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Anillin in NIH/3T3 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Anillin in human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Anillin in HUVEC and HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Anillin (UniProt: Q9NQW6; also known as ANLN, Anillin acting binding protein) is encoded by the ANLN gene (Gene ID: 54443) in human. Anillin is a scaffold protein that links the actomyosin ring to membrane and RhoA. It is ubiquitously expressed with high levels found in the brain, placenta, and testis. It is also overexpressed in many tumor types including breast, colorectal, hepatic, pancreatic, and kidney tumors. In dividing cells, Anillin expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter the G1 phase. It is mainly found in the nucleus during interphase and colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis. Subsequently it concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. Anillin is shown to be essential for cytokinesis and it maintains the structural integrity of the cleavage furrow. Anillin contains a conserved N-terminal actin and myosin binding domains, an Anillin homology domain and PH domain at the C terminus that is required for robust furrow localization and for its function. Anillin is reported to both enhance and limit contractility during cytokinesis through binding directly to active RhoA as well as several proteins that positively or negatively regulate Rho, and by crosslinking F-actin. Anillin is shown to be highly phosphorylated in mitosis and serves as a substrate for mitotic kinases. In human cells Anillin can be phosphorylated on 46 different residues. Phosphorylation at serine 635 is a key to mediate cytokinesis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Piekny, AJ., and, Glotzer, M. (2008). Curr. Biol. 18(1); 30-36; Kim, H., et al. (2017). PLoS Genet 13(1); e1006511; Arnold, TR., et al., (2019). eLife.8; e39065).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

0.03 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2