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X2252

Sigma-Aldrich

黄嘌呤氧化酶 微生物

lyophilized powder, ≥7 units/mg solid

同義詞:

XO, 次黄嘌呤氧化酶, 黄嘌呤氧化酶, XOD, 黄嘌呤:氧化还原酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

microbial

形狀

lyophilized powder

比活性

≥7 units/mg solid

分子量

~160 kDa

技術

inhibition assay: suitable

顏色

brown

最適pH

7.5-8.0

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)

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一般說明

研究领域:细胞周期

黄嘌呤氧化酶(XO),一种多功能的含钼黄素蛋白,是黄嘌呤氧化还原酶(XORs)的形式之一。每个黄嘌呤氧化酶由2个黄素分子(FAD)、2个钼原子和8个铁原子构成。[1]

應用

黄嘌呤氧化酶(微生物)可用于:
  • 测定体外黄嘌呤氧化酶活性[2]
  • 在超氧化物歧化酶检测中生成超氧阴离子[3]
  • 作为比色纸装置的反应混合液组分,酶法检测传统药物中的别嘌呤醇[4]

生化/生理作用

还含有蛋白水解活性。
黄嘌呤氧化酶在嘌呤代谢中催化次黄嘌呤羟基化为黄嘌呤,黄嘌呤羟基化为尿酸和氧自由基。[3][5]这种酶也参与咖啡因代谢。[6]黄嘌呤氧化酶在药物等异源物质的代谢中也起重要作用,例如硫嘌呤类药物,涉及6-巯基嘌呤、别嘌呤醇和尿酸。[7]黄嘌呤氧化酶活性过高会引起高尿酸血症(血中尿酸过多),进一步导致痛风。[8]

物理性質

抑制剂:还原剂,Hg++,Ag+,MIA
最佳pH: 7.5 - 8.0
最佳温度:65oC
pH稳定性:pH 6.5 - 9.0 (25oC, 15hr)
温度稳定性:低于55oC (pH 8.0, 30min)

單位定義

在25 °C pH 7.5条件下,一个单位可在每分钟内将1.0 μ摩尔的黄嘌吟转化为尿酸。在次黄嘌呤作为底物时可获得约50%的活性。

外觀

含有BSA和谷氨酸钠作为稳定剂的冻干粉末

儲存和穩定性

密闭。干燥。上锁保存或放置在只有经过授权或有资质的
人员才能进入的区域。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Giuseppe Faudone et al.
Journal of medicinal chemistry, 64(11), 7156-7178 (2021-05-22)
The purine alkaloid caffeine is the most widely consumed psychostimulant drug in the world and has multiple beneficial pharmacological activities, for example, in neurodegenerative diseases. However, despite being an extensively studied bioactive natural product, the mechanistic understanding of caffeine's pharmacological
Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
The cytotoxicity of five oxysterols identified in cooked fish, 7-ketocholesterol, 7beta-hydroxycholesterol, cholesterol 5alpha,6alpha-epoxide, cholestanetriol and 4-cholesten-3-one, was investigated in primary cultures of bovine ovarian granulosa cells. Cells were exposed to the oxysterols individually and to a mixture of the same
COLORIMETRIC PAPER-BASED DEVICE BY ENZYMATIC REACTION FOR DETECTING ALLOPURINOL IN TRADITIONAL MEDICINE
Rimadani P, et al.
Rasayan Journal of Chemistry (2021)

條款

The appearance of uric acid is measured at 293nm by spectrophotometry.

相關內容

The appearance of uric acid is measured at 293nm by spectrophotometry.

Questions

1–2 of 2 Questions  
  1. Good morning, I purchased xanthine oxidase X2252-25UN for quantifying SOD enzymatic activity. Which buffer should I use to solubilize the enzyme? After solubilization, how should I store the resulting solution?

    1 answer
    1. This product is soluble in water at a concentration of 10 mg/mL. It can also be dissolved in a buffer according to specific experimental references, such as 50 mM potassium phosphate. The stability of the solution has not been tested.

      Helpful?

  2. Guten Tag, in welchem Lösungsmittel und bis zu welcher Konzentration ist das Enzym gut löslich? Kann Natrium Phosphat Puffer verwendet werden? Danke.

    1 answer
    1. For assay purposes, this product is prepared in 50 mM Potassium Phosphate Buffer, pH 7.5 at 25°C. The material is lyophilized from a solution containing BSA and sodium glutamate. While the solubility has not specifically been tested in sodium phosphate buffer, it is expected to be suitable. Please see the link below to review the assay protocol for this item:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/938/x2252enz.pdf

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