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重要文件

U1875

Sigma-Aldrich

尿素酶 来源于洋刀豆 (刀豆)

Type III, glycerol solution, 500-800 units/mL

同義詞:

Jack bean urease, Urea amidohydrolase

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

Canavalia ensiformis

種類

Type III

形狀

glycerol solution

分子量

~544620

濃度

500-800 units/mL

儲存溫度

2-8°C

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一般說明

亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
洋刀豆(直生刀豆)的尿素酶是一种含有840个氨基酸残基的单链六聚体,存在于植物、细菌、真菌、藻类和土壤中。它被视为是第一种含镍的酶,也是第一种结晶化酶。尿素酶以多种异构体存在,部分包括洋刀豆尿素酶(JBURE-I)、刀豆毒素(CNTX)和JBURE-IIB。JBURE-I比其他两种异构体更加丰富。尿素酶存在成熟的种子中。

應用

来自直生刀豆(洋刀豆)的尿素酶已用于:
  • 使用从左心室(LV)和室间隔组织中提取的样本测定肌酸和磷酸肌酸
  • 测定稳定尿样中尿素的浓度
  • 一种标准品,以测定植物提取物的尿素酶活性

生化/生理作用

在植物中,尿素酶对于自然界中氮的生物利用度/代谢和防御机制至关重要。尿素酶是一种用于催化尿素水解的金属酶,以释放生长所需氮源—氨以及二氧化碳。他们表现内部肽介导的杀虫作用和杀菌活性。
脲酶活性已显示在暴露于光后被苯并芘抑制。这种抑制作用是由于苯并芘对脲酶′巯基的作用。 [1]

單位定義

一微摩尔单位是指在pH 7.0,25℃下每分钟催化尿素释放1.0 μmole NH3所需的酶活性。它相当于1.0 I.U.或0.054 Sumner单位(在pH 7.0,20℃下,5分钟内产生1.0 mg氨氮)

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Fernando Medina Ferrer et al.
Geobiology, 20(1), 79-97 (2021-08-03)
Modern carbonate tufa towers in the alkaline (~pH 9.5) Big Soda Lake (BSL), Nevada, exhibit rapid precipitation rates (exceeding 3 cm/year) and host diverse microbial communities. Geochemical indicators reveal that carbonate precipitation is, in part, promoted by the mixing of calcium-rich
Melissa Postal et al.
Peptides, 38(1), 22-32 (2012-08-28)
Ureases (EC 3.5.1.5) are metalloenzymes that hydrolyze urea into ammonia and CO(2). These proteins have insecticidal and fungicidal effects not related to their enzymatic activity. The insecticidal activity of urease is mostly dependent on the release of internal peptides after
Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Fernando Medina Ferrer et al.
Microbial biotechnology, 13(6), 1877-1888 (2020-07-29)
Microbial precipitation of calcium carbonate is a widespread environmental phenomenon that has diverse engineering applications, from building and soil restoration to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate
Pınar Tekiner et al.
Journal of molecular recognition : JMR, 25(11), 549-554 (2012-10-31)
Jack bean (Canavalia ensiformis) is the source of interesting proteins that contribute to modern biochemistry, and urease is the primary of these proteins. Owing to its role and occurrence in nature, urease has become a part of extensive studies. In

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Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Questions

  1. How much glycerol is in Urease product no. U1875?

    1 answer
    1. The specific concentration of glycerol is considered proprietary, but will range from 50 - 70%.

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