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GE27-0846-01

Thrombin Protease

Cytiva 27-0846-01

同義詞:

Proteolytic Enzyme, Thrombin Activity, Thrombin Enzyme

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.56
暫時無法取得訂價和供貨情況

包裝

pkg of 500 units

製造商/商標名

Cytiva 27-0846-01

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma.

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors (see pGEX Vectors (GST Gene Fusion System)) containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

應用

Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

特點和優勢

  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

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條款

本頁說明與 Cytiva 產品搭配使用的 pGEX 表達載體的特性。

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.

相關內容

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

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