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一般說明
来自粘质沙雷氏菌的内切酶是一种二聚体，包含两个具有不同蛋白质折叠的相同单体。核心包含一个反平行的六股β-片，两侧各有α-螺旋。每个单体具有一个活性位点。这种酶是一种依赖镁的核酸酶。
應用
来自粘质沙雷氏菌的碳化切酶已被用于邻近生物素化分析(BioID)和亲和纯化期间的细胞裂解。它也被用作裂解缓冲液的组分，以从细胞系中提取蛋白，用于亲和纯化研究。
碳化切酶在一项研究中用于评估TY3 gag3间隔物对细胞内冷凝和脱壳的影响。
用于去除蛋白样品中的核酸。
生化／生理作用
涡轮核酸切酶通过降低粘度和降解RNA、基因组DNA、杆状病毒DNA和未包壳载体DNA提供核酸酶处理。
粘质沙雷氏菌的核酸内切酶对单链和双链DNA和RNA均有效。它介导3个 O—P键的消化，产生以5个单磷酸结尾的寡核苷酸。已知这种酶的活性较少受还原剂和潮剂的影响。它在室温下高度稳定。这种内切酶在下游处理过程中可消除不需要的核酸。
消化天然或热变性的DNA和RNA。
單位定義
在pH 8.0，37 °C条件下，一个单位的酶可在30分钟内将超声鲑鱼精子DNA消化成酸溶性寡核苷酸，等同于ΔA260的1.0。
外觀
以溶于50mM Tris-HCl (pH 8.0)和50 mM NaCl的溶液形式提供。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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