推薦產品
生物源
Streptomyces luteosporeus
品質等級
化驗
≥95% (HPLC)
形狀
solid
溶解度
DMSO: 0.90 - 1.10 mg/ml, clear, yellow
抗生素活性譜
fungi
作用方式
enzyme | inhibits
運輸包裝
wet ice
儲存溫度
−20°C
SMILES 字串
CCC(=O)NC1=C2SSC=C2N(C)C1=O
InChI
1S/C9H10N2O2S2/c1-3-6(12)10-7-8-5(4-14-15-8)11(2)9(7)13/h4H,3H2,1-2H3,(H,10,12)
InChI 密鑰
UGZYFXMSMFMTSM-UHFFFAOYSA-N
應用
生化／生理作用
Sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. It was found to inhibit in vitro RNA synthesis directed by all three yeast RNA polymerases (I, II, and III). Thiolutin is also an inhibitor of mannan and glucan formation in Saccharomyces cerevisiae and used for the analysis of mRNA stability. Studies have shown that thiolutin inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin and thus suppresses tumor cell-induced angiogenesis in vivo.
準備報告
Thiolutin dissolves in DMSO at 0.90 - 1.10 mg/ml to yield a clear, yellow solution.
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 2 Oral
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
N Sivasubramanian et al.
Molecular & general genetics : MGG, 180(3), 609-615 (1980-01-01)
Two mutations in Escherichia coli conferring resistance to the transcription initiation inhibitor, thiolutin, have been mapped. One of these mutations (tln-I)( maps at 10.2 min on the genetic map and is cotransducible with dnaZ at a frequency of approximately 50%.
Vicent Pelechano et al.
Yeast (Chichester, England), 25(2), 85-92 (2007-10-05)
Thiolutin is commonly used as a general inhibitor of transcription in yeast. It has been used to calculate mRNA decay rates by stopping the transcription and then determining the relative abundance of individual mRNAs at different times after inhibition. We
Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast
Gutierrez H, et al.
Microbial cell, 5(11), 511-511 (2018)
Jeff Coller
Methods in enzymology, 448, 267-284 (2008-12-30)
Much of our understanding of eukaryotic mRNA decay has come from studies in budding yeast, Saccharomyces cerevisiae. The facile nature of genetic and biochemical manipulations in yeast has allowed detailed investigations into the mRNA decay pathway and the identification of
T Kadowaki et al.
The Journal of cell biology, 126(3), 649-659 (1994-08-01)
To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16
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