SRP6272

SPAM1 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

• 同義詞: HYA1, HYAL1, HYAL3, HYAL5, PH-20, SPAG15
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 共軛: (Glycosaminoglycan)
• 籤條: 6-His tagged (C-terminus)
• 化驗: ≥92% (SDS-PAGE)
• 形狀: lyophilized powder
• 分子量: calculated mol wt 52.1 kDa; observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.)
• 包裝: pkg of 10 μg; pkg of 50 μg
• 雜質: <1 EU/μg endotoxin (LAL test)
• UniProt登錄號: P38567
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... SPAM1(6677)

描述

一般說明

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

外觀

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable