SRP0229

WWP1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)

• 同義詞: AIP5, Atrophin-1 interacting protein 5, NEDD4-like E3 ubiquitin-protein ligase, TGIF-interacting ubiquitin ligase 1 (Tiul1), WW domain containing E3 ubiquitin protein ligase 1
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥15% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 106 kDa
• 包裝: pkg of 20 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_007013
• UniProt登錄號: Q9H0M0
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... WWP1(11059)

描述

一般說明

Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.