Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system.
生化／生理作用
Useful for the study of enzyme kinetics, screening inhibitors.
單位定義
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Histone-based chromatin organization paved the way for eukaryotic genome complexity. Because of their key role in information management, the histone posttranslational modifications (hPTM), which mediate their function, have evolved into an alphabet that has more letters than there are amino
