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Merck
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重要文件

SAE0195

Sigma-Aldrich

IceTEV Protease

Histidine tagged recombinant protein, aqueous solution

同義詞:

TEV protease

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

品質等級

形狀

aqueous solution

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

TEV protease is a highly sequence specific serine protease from Tobacco Etch Virus. Its high specificity makes TEV protease popular for cleaving recombinant fusion proteins. However, at low temperatures such as 4 °C, standard TEV Protease has only ~10% of its activity compared to regular temperature conditions (such as 25-30 °C). IceTEV Protease is a new TEV Protease version that is designed to cleave fusion proteins effectively at low temperatures also, in the range of 2-10 °C, with comparable efficiency to cleavage by standard TEV Protease at 25-30 °C. Following the cleavage reaction, IceTEV Protease can be removed from the reaction mixture with nickel-agarose (Ni-agarose) beads.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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文章

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

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