推薦產品
重組細胞
expressed in E. coli
化驗
≥95% (SDS-PAGE)
形狀
aqueous solution
比活性
≥1000 units/mg protein
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
D-2-羟基戊二酸脱氢酶(D2HGDH)是D-2-羟基酸NAD+依赖性脱氢酶家族的成员。D2HGDH催化α-酮戊二酸(α-KG)转化为D-2-羟基戊二酸(D2HG),并与NADH氧化为NAD+偶联。
已经报道了来自发酵酸氨基球菌的D2HGDH的晶体结构。来自发酵酸氨基球菌的D2HGDH已用于几种酶促测定,例如:
已经报道了来自发酵酸氨基球菌的D2HGDH的晶体结构。来自发酵酸氨基球菌的D2HGDH已用于几种酶促测定,例如:
- 基于酮化合物和L-谷氨酸的转氨作用,测量氨基转移酶活性的连续分光光度测定法,其产生相应的氨基化合物和2-氧代戊二酸酯。
- 测定生物体液(例如血清,尿液,细胞培养上清液以及细胞或组织裂解液)中的D2HG水平。
- 用于测量支链氨基酸氨基转移酶活性的偶联测定系统。
單位定義
一个单位的酶将1μmole NADH氧化为NAD+,并在37℃,pH 8.0下每分钟将α-酮戊二酸还原为(D)-2-羟基戊二酸。
準備報告
该重组D2HGDH产品以水溶液的形式提供在20 mM Trizma®缓冲液(pH 7.5)中,包含150 mM NaCl和10%甘油。
法律資訊
T3P is a registered trademark of Archimica GmbH
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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