Upon verification, the laboratory has not characterized the biological activity of SAE0089. However, it is known to be reversibly inactivated by oxidation, where the active form is present in a reduced format. Therefore, just prior to application, one could activate it by incubation with DTT for about 15 minutes at 37 degrees Celsius.
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一般說明
Streptolysin O (SLO)是一种免疫原性、氧不稳定的溶血性外毒素，可通过二硫苏糖醇可逆性激活。[1]在大多数A组菌株以及C组和G组链球菌的许多菌株生长过程中，它与包括streptolysin S在内的其他毒素一起释放到细胞外培养基中。[1,2] SLO和Streptolysin S的区别在于SLO具有免疫原性和氧不稳定性，而Streptolysin S具有氧稳定性和非免疫原性，只有与载体蛋白相关时才有活性[3] SLO的溶血活性是通过在含胆固醇脂质膜上形成多聚纳米孔介导的。
SLO 可用于细胞透化或溶血。不同动物种属的红细胞对 SLO 溶血的敏感性差异显著。[1]使用 SLO 对多种细胞类型和各种应用进行了细胞透化。例如，将它用来将反义寡核苷酸导入培养的真核细胞；[3]探讨鸟嘌呤核苷酸类似物对人 T 淋巴细胞磷脂酰肌醇代谢及蛋白激酶 C （PKC）活化的影响；[4]监测膜脂双层内的胆固醇氧化；[5] 并使用外部荧光团标记活细胞内的蛋白质。[6]
SLO 可用于细胞透化或溶血。不同动物种属的红细胞对 SLO 溶血的敏感性差异显著。[1]使用 SLO 对多种细胞类型和各种应用进行了细胞透化。例如，将它用来将反义寡核苷酸导入培养的真核细胞；[3]探讨鸟嘌呤核苷酸类似物对人 T 淋巴细胞磷脂酰肌醇代谢及蛋白激酶 C （PKC）活化的影响；[4]监测膜脂双层内的胆固醇氧化；[5] 并使用外部荧光团标记活细胞内的蛋白质。[6]
應用
透膜允许细胞摄取大分子或带电分子。
生化／生理作用
本品在大肠杆菌中重组表达而成，含有SLO的完整天然蛋白序列(Uniport ID: P0DF96 aa 34-571)，未添加任何纯化标记，计算分子量为60,144 Dalton。材料由含有20 mM钠盐Hepes pH 7.5、150 mM氯化钠和2 mMEDTA的溶液冻干。
能穿透动物细胞膜的巯基活化毒素。
能穿透动物细胞膜的巯基活化毒素。该蛋白作为单体与膜胆固醇结合，随后聚合成围绕>12 nm孔的大弧形和环形结构。
單位定義
一个单位将导致 50 μL 的 2% 人红细胞悬液在 37 °C的磷酸盐缓冲盐水（pH 7.4） 中发生50%溶解。
含 Hepes 缓冲盐和 EDTA的冻干粉。
訊號詞
Danger
危險分類
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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分析證明 (COA)
Lot/Batch Number
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A method of membrane permeabilization of T lymphocytes with the bacterial cytotoxin streptolysin O has allowed the effect of guanine nucleotide analogues on phosphatidylinositol metabolism and protein kinase C (PKC) activation to be investigated. The data demonstrate that, in permeabilized
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Cellular uptake of antisense oligonucleotides is critical to their ability to inhibit gene expression. In the present study, phosphodiester oligodeoxynucleotides were introduced into cells during brief permeabilization with the pore-forming agent streptolysin O. The extent of antisense inhibition was dependent
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Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a
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I purchased SAE0089 (streptolysin o). I see in many protocols and one of your datasheets that Streptolysin o should be treated with 10 mM dithiothreitol before using it. However, I don't see that indication anywhere for SAE0089. Do I still need to treat it with DTT?
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