推薦產品
生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~49 kDa
物種活性
rat, mouse, human
濃度
~1.0 mg/mL
技術
immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.
western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... WIPI2(26100)
mouse ... Wipi2(74781)
rat ... Wipi2(288498)
一般說明
The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif.
WD repeat domain, phosphoinositide-interacting 2 (WIPI-2) belongs to the WIPI protein family. It is localized on the autophagy membrane and plasma membrane. Apart from this, it is also present on the membranes near the Golgi cisternae. WIPI2 is a mammalian ortholog of Atg18, which is ubiquitously expressed in a variety of cell lines.
特異性
Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2.
應用
Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
生化／生理作用
WIPI-2 (WD repeat domain, phosphoinositide-interacting 2) plays a vital role in the transformation of omegasomes into autophagosomes. The above protein functions as a mammalian effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI2 aids recombinant capsid protein VP1 (rVP1) in upregulating autophagy, MAPK1 (Mitogen-activated protein kinase 1)/3 phosphorylation and matrix metallopeptidase 9 (MMP-9) activity, which are required for macrophage migration.
外觀
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Recombinant protein rVP1 upregulates BECN1-independent autophagy, MAPK1/3 phosphorylation and MMP9 activity via WIPI1/WIPI2 to promote macrophage migration.
Liao CC
Autophagy (2013)
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Polson HE
Autophagy (2010)
Melanie Haas Kucherlapati
Oncotarget, 10(65), 6913-6933 (2019-12-21)
Genes of the pre-replication, pre-initiation and replisome complexes duplicate the genome from many sites once in a normal cell cycle. This study examines complex components in lung adenocarcinoma (LUAD) closely, correlating changes in the genome and transcriptome with proliferation and
Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.
Proikas-Cezanne T
Journal of Cellular and Molecular Medicine (2011)
Wei Wan et al.
Molecular cell, 72(2), 303-315 (2018-10-20)
mTORC1, the major homeostatic sensor and responder, regulates cell catabolism mainly by targeting autophagy. Here, we show that mTORC1 directly controls autophagosome formation via phosphorylation of WIPI2, a critical protein in isolation membrane growth and elongation. mTORC1 phosphorylates Ser395 of
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