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S5442

Sigma-Aldrich

SyntheChol ® NS0 补充

SyntheChol® NS0 Supplement

500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

同義詞:

胆固醇 溶液

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About This Item

經驗公式(希爾表示法):
C27H46O
CAS號碼:
分子量::
386.65
MDL號碼:
分類程式碼代碼:
12352211
eCl@ss:
39023139
PubChem物質ID:
NACRES:
NA.75
暫時無法取得訂價和供貨情況

生物源

non-animal source

無菌

sterile-filtered

產品線

BioXtra

形狀

aqueous solution

濃度

500 ×

技術

cell culture | mammalian: suitable

儲存溫度

2-8°C

SMILES 字串

[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C

InChI

1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1

InChI 密鑰

HVYWMOMLDIMFJA-DPAQBDIFSA-N

基因資訊

尋找類似的產品? 前往 產品比較指南

應用

SyntheChol® NS0补充剂可用作NS0小鼠骨髓瘤细胞生长和其他胆固醇依赖性细胞系生长的培养基补充剂。[1][2][3][4][5]

特點和優勢

合成的非动物胆固醇溶液,作为杂交瘤细胞培养基制剂的补充剂,可促进NS0小鼠骨髓瘤和其他胆固醇依赖性细胞系的良好生长和蛋白质生产。

法律資訊

SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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From our recent publications, it was found that the deimmunization method (Dharshanan et al. (2012) Sci Res Essays 7:2288-2299) should be applied for the development of humanized anti-C2 monoclonal antibody (H1C2 mAb). However, the overlapping-PCR mutagenesis procedure used to insert
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文章

Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.

Questions

1–4 of 4 Questions  
  1. What is the concentration of product S5442?

    1 answer
    1. Unfortunately, the formulation of Product S5442 is considered proprietary.

      Helpful?

  2. Is this formulation stable at room temperature? If so, how long is it stable?

    1 answer
    1. This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  3. Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?

    1 answer
    1. The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  4. Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you

    1 answer
    1. Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.

      Helpful?

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