R5279

Anti-Rab9 from rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-Ras-Associated Protein Rab9, Anti-Ras-Related Protein Rab9
• MDL號碼: MFCD03097193
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~23 kDa
• 物種活性: rat, mouse, human
• 濃度: ~1 mg/mL
• 技術: indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin; western blot: 5-10 μg/mL using whole extract of human A431 cells
• UniProt登錄號: P51151
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RAB9A(9367) ; mouse ... Rab9(56382) ; rat ... Rab9a(84589)

描述

一般說明

Rab9 is a member of the Rab family of guanosine triphosphatases (GTPases). Rab9 GTPase is localized predominantly to late endosomes. Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif implicated in subcellular targeting.

免疫原

synthetic peptide corresponding to amino acid residues 188-200 of human Rab9, conjugated to KLH.

應用

Anti-Rab9 from rabbit has been used in immunoblotting and immunofluorescence.

生化／生理作用

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the trans-Golgi network (TGN). Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein TIP47 (tail-interacting protein of 47 kDa). This interaction is required for Rab9 stability on late endosomes. Anti-Rab9 antibodies may be used as a marker for late endosomes.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable