R4752

Ribonucleic acid, transfer from bovine liver

Type XI, lyophilized powder

• 同義詞: Transfer RNA, tRNA
• CAS號碼: 9014-25-9
• MDL號碼: MFCD00132200
• 分類程式碼代碼: 41106305
• NACRES: NA.51

屬性

• 種類: Type XI
• 形狀: lyophilized powder
• 儲存溫度: −20°C

描述

一般說明

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure.[1] It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.[2]

應用

Ribonucleic acid, transfer from bovine liver has been used:

• to analyze the efficiency of thioflavin T (ThT) as a fluorophore[3]
• to test its ability to inhibit the growth of endothelial cells[4]
• as a component in translation mixture[5]
• as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column[6]

Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

生化／生理作用

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein.[1] There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain.[2] It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.[1]

單位定義

一单位在 1.0mL 水（1cm 光程）中的 A₂₆₀ 为 1.0。

分析報告

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)