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10108626001

Roche

Poly(A)

lyophilized, suitable for PCR, pkg of 100 mg

同義詞:

Poly(A), 聚腺苷酸

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About This Item

分類程式碼代碼:
41105600

描述

Polyadenylic acid

品質等級

形狀

lyophilized

分子量

700-3500 kDa

包裝

pkg of 100 mg

製造商/商標名

Roche

濃度

0.5 mg/mL (Working concentration)

技術

PCR: suitable

顏色

white

溶解度

water: soluble

吸光率

A290/260 nm 0.03-0.05
A280/260 nm 0.28-0.32
A250/260 nm 0.86-0.90

儲存溫度

2-8°C

一般說明

Poly(A)被用作DNA和RNA定量沉淀的载体。

應用

Poly(A)已可用于微滴式数字PCR(ddPCR)分析。
聚腺苷酸Poly(A)抑制 Exo1的核酸外切酶活性。还用作重悬冻干 gBlocks 的载体。

生化/生理作用

聚腺苷酸(Poly(A))尾部有3′末端,在细胞核产生。哺乳动物细胞中,约含 250 个核苷酸。Poly(A)可调控mRNA降解和翻译起始。胞质Poly(A)的延伸调控翻译过程。

品質

典型分析:
2.3μmol/mg Poly(A)(来自吸光度)相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A250/A260、A280/A260、A290/A260

序列

链长为 2.100 至 10.000 个核苷酸

單位定義

1个A260 单位相当于40 μg ssRNA。

外觀

冻干品,钾盐

準備報告

工作浓度:0.5 mg/ml
建议浓度为0.5 mg/ml。
工作溶液:建议浓度为0.5 mg/ml。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
Kengo Watanabe et al.
Nature communications, 12(1), 1353-1353 (2021-03-03)
Cells are under threat of osmotic perturbation; cell volume maintenance is critical in cerebral edema, inflammation and aging, in which prominent changes in intracellular or extracellular osmolality emerge. After osmotic stress-enforced cell swelling or shrinkage, the cells regulate intracellular osmolality
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in

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