推薦產品
等級
for molecular biology
形狀
buffered aqueous glycerol solution
濃度
≥5000 units/mL
5,000 units/mL
運輸包裝
wet ice
儲存溫度
−20°C
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特異性
Recognition sequence: 5′-CGAT/CG-3′
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
應用
PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.
其他說明
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
外觀
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 300 mM KCl, 1 mM dithioerythritol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Sanbing Shen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(43), 10893-10904 (2008-10-24)
Disrupted-in-Schizophrenia-1 (DISC1), identified by positional cloning of a balanced translocation (1;11) with the breakpoint in intron 8 of a large Scottish pedigree, is associated with a range of neuropsychiatric disorders including schizophrenia. To model this mutation in mice, we have
E Bonnelye et al.
The Journal of cell biology, 153(5), 971-984 (2001-05-31)
The orphan nuclear estrogen receptor-related receptor alpha (ERRalpha), is expressed by many cell types, but is very highly expressed by osteoblastic cells in which it transactivates at least one osteoblast-associated gene, osteopontin. To study the putative involvement of ERRalpha in
T R Gingeras et al.
Nucleic acids research, 9(18), 4525-4536 (1981-09-25)
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5'
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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