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M7027

Sigma-Aldrich

甲基纤维素

viscosity: 15 cP, BioReagent, suitable for cell culture

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About This Item

CAS號碼:
9004-67-5
MDL號碼:
MFCD00081763
分類程式碼代碼:
12352201
PubChem物質ID:
24897135
NACRES:
NA.75
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生物源

synthetic (organic)

產品線

BioReagent

形狀

powder

技術

cell culture | mammalian: suitable

黏度

12-18 cP, 2 % in H2O(20 °C)

溶解度

water: 50 mg/mL, clear to hazy

SMILES 字串

[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]

InChI 密鑰

YLGXILFCIXHCMC-JHGZEJCSSA-N

尋找類似的產品？ 前往 產品比較指南

一般說明

甲基纤维素由纤维素醚化而得。这种醚类纤维素商品具有两亲性及原本的理化特性。甲基纤维素抗酶解、抗消化、无过敏性、无毒性。

應用

甲基纤维素可用于制备半固体基质,[1][2] 用作细胞培养凝胶，用于培养集落形成细胞[3][4] （如CFU-G、CFU-GM）和用于毒性检测的其他细胞。粘度低，不适用于噬菌斑检测或克隆。
甲基纤维素可用于：
  • 在经口管饲法中作为药物二甲双胍的溶剂
  • 在乳腺球培养中，作为乳腺上皮细胞生长培养基的组分
  • 在悬滴法球状体侵袭试验中，作为球状体三维共培养补充剂

生化／生理作用

甲基纤维素广泛应用多个行业，在食品业中可用作乳化剂、质感剂、增稠剂和胶凝添加剂。甲基纤维素也可用于各种化妆品和个人护理用品，例如洗发水、头发造型用品、液体皂、沐浴露、乳霜、牙膏，使稠度均匀。此外，它也可作为药物添加剂，生产营养补充剂胶囊。甲基纤维素可替代冻存液中的甘油。它可与培养基添加剂共同作用，增强多系或单系造血祖细胞的增殖和分化。这种纤维素衍生物还可用于防止多种细胞的粘附能力和细胞功能丧失，包括人皮肤成纤维细胞、人黑色素细胞和小鼠肺成纤维细胞悬液。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000434092
0000434092
0000409636
0000395387
0000395386

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存取文件庫

Large variability in the motility of spiroplasmas in media of different viscosities
Boudet JF, et al.
Scientific Reports, 8(1), 17138-17138 (2018)
Chandler Warr et al.
ACS applied bio materials, 3(4), 2239-2244 (2020-05-30)
We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol)
High throughput and automatic colony formation assay based on impedance measurement technique
Lei KF, et al.
Analytical and Bioanalytical Chemistry, 409(12), 3271-3277 (2017)
Barley beta-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts
Fuste NP, et al.
Carbohydrate Polymers, 389-398 (2019)
Dissociated effect and Chemosensitive enhancement of tumor spheroids influenced by an electric field in a microdevice
Huang Chun-Hao, et al.
Biomedical Microdevices, 20(3), 70-70 (2018)
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條款

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

造血幹細胞培養方法

CD34+細胞的分離、擴大和分化的造血幹細胞培養方案，包括CFU測試。

Questions

1–10 of 13 Questions  

  1. How to practically reach a 1% solution of Methylcellulose in cell culture media that can't be heated excessively?

    1 answer

    1. This product is supplied in powder form. The solubility in water is 50 mg/mL, resulting in a hazy solution. To prepare a 1% Methylcellulose solution in cell culture media from this stock solution, follow these steps:
      A 1% solution requires 1 g of solute per 100 mL of solution, equivalent to 10 mg/mL. The stock solution is 50 mg/mL. To achieve this, take 20 mL of the 50 mg/mL stock solution and dilute it to a total volume of 100 mL.

      Helpful?

  2. I would like to use methylcellulose in my cell culture media to optimize organoids in suspension. What methylcellulose product would be the most appropriate? And can I dissolve it directly into the cold media?

    1 answer

    1. The methylcellulose products available are not currently tested for use in organoid suspension. However, a previously available product, S3812, was an IMDM-based stem cell culture medium that included a methylcellulose component with a 4000 cps viscosity, at a concentration of 1%. This would correspond to product M0512. The solubility in cold medium has not been determined. Please see the link below to review the general methylcellulose datasheet which includes a viscosity comparison table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/010/m6385pis.pdf

      Helpful?

  3. Can the M7027 pass through a 0.2um filter?

    1 answer

    1. Methyl cellulose has a high molecular weight, and the viscosity of methyl cellulose solutions can also impede its ability to pass through such fine filters, as it can clog the filter pores. For sterilization, a 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of solution.

      Helpful?

  4. Does this product need to be sterilized after dissolving for use in cell culture?

    1 answer

    1. Solutions of this material require sterilization for cell culture applications. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of the solution.

      Helpful?

  5. Hello sir, I need to know molar mass or molecular weight of this code of Methyl cellulose or tell me how can i calculate can you help me?

    1 answer

    1. The molecular weight is not analyzed on a lot to lot basis. The approximate molecular weight is 88,000 Da with methoxy substitution between 27.5 - 31.5% (weight).

      Helpful?

  6. Can I use this to make 0.5% solution in water which will be used as the solvent to dissolve neratinib and administrate to mice through oral gavage?

    1 answer

    1. This product can be solubilized in water at 50mg/ml which is a 0.5% solution. This product is tested for use in cell cultue, however it has not been tested for in vivo applications. Suitability for in vivo use will need to be validated by the end-user. See the link below for a general product information sheet for Methyl Cellulose. Note product M7140, which is the general research grade of this product. All details for M7140 will apply to M7027.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/785/m0512pis.pdf

      Helpful?

  7. Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?

    1 answer

    1. Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media.  Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.

      Helpful?

  8. How do I store a stock solution of Methyl cellulose, Product M7027?

    1 answer

    1. A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How can I make a solution of Methyl cellulose, Product M7027?

    1 answer

    1. There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.

      Helpful?

1–10 of 13 Questions  

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