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PLX15

Sigma-Aldrich

GenElute 质粒大量制备试剂盒

greener alternative

sufficient for 15 purifications

同義詞:

Gen Elute, GenElute 质粒试剂盒

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 15 purifications

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA purification: suitable

環保替代類別

儲存溫度

15-25°C

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一般說明

GenElute 质粒大量制备试剂盒为简单、快速、低成本的从大肠杆菌培养物中分离质粒DNA。该试剂盒结合了硅胶结合技术和离心柱形式的便利性,可在45分钟内从25-200 ml LB培养基中回收出多达1.2mg的质粒DNA。请注意,实际得率和最佳的培养物体积取决于质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物,并通过改性SDS碱裂解法进行处理,之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后,在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。

Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 (The 12 Principles of Greener Chemistry),更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法,本产品符合固有安全化学原则(Inherently Safer Chemistry)。

應用

回收的质粒DNA主要以超螺旋形式存在。用琼脂糖凝胶电泳没有检测出可见的基因组DNA或RNA污染。回收的DNA可直接用于下游应用使用,如限制性酶切,连接,测序,PCR和转染。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Han Liu et al.
FEBS letters, 583(14), 2365-2371 (2009-06-24)
The chaperonin GroEL contains two seven-subunit rings, and allosteric signals between them are required to complete the GroEL reaction cycle. For this reason SR1, a mutant of GroEL that forms only single rings, cannot function as a chaperone. Mutations in
Characterisation of mutations in GroES that allow GroEL to function as a single ring.
Liu H
Febs Letters, 583(14), 2365-2371 (2009)
Martina Herrmann et al.
Applied and environmental microbiology, 75(10), 3127-3136 (2009-03-24)
Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and

條款

The product bulletin providin detailed use protocol for easy DNA transfection.

Product manual provides detailed protocol for easy DNA transfection.

相關內容

Product manual provides detailed protocol for easy DNA transfection.

The product bulletin providin detailed use protocol for easy DNA transfection.

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