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Merck
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重要文件

PHG0002

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TRIS盐酸盐

同義詞:

Trizma® 盐酸盐 盐酸盐, TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺

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About This Item

線性公式:
NH2C(CH2OH)3 · HCl
CAS號碼:
分子量::
157.60
Beilstein:
3675235
EC號碼:
MDL號碼:
分類程式碼代碼:
12161700
NACRES:
NA.21
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生物源

synthetic

形狀

powder

技術

cell culture | mammalian: suitable

雜質

Endotoxin, microbial, and trace metals; tested

有用的pH值範圍

7.0-9.0

pKa (25 °C)

8.1

適合性

suitable for manufacturing use

異物活動

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES 字串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI 密鑰

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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應用

Tris HCl 是一种常用的生物缓冲液。Tris/Tris HCl 作为缓冲系统在生物制药生产、细胞培养、诊断和分子生物学等生物系统中具有非常广泛的用途。Tris 的 pKa 为 7.77,与生理 pH 值 7.36 非常接近。

Tris HCl 作为 Tris/氨丁三醇/Trizma 的偶联缓冲液组分用于生物制药缓冲液配方(上下游应用)、电泳分离和诊断试剂配方。

其他說明

所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液,温度每降低 1°C,pH 就会增加约 0.03 单位,每稀释 10 倍,pH 会降低 0.03-0.05 单位。对于一些精准的应用,请使用经过仔细校准的pH计和玻璃/甘汞组合电极。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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