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PEROX1

Sigma-Aldrich

过氧化物酶体分离试剂盒

isolate peroxisomes from tissues and cells

同義詞:

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

一般說明

分离所得的过氧化物酶体可用于研究脂质β-氧化、氨基酸代谢及醚链连接的甘油糖脂和胆汁酸的生物合成。

應用

过氧化物酶体分离试剂盒提供了使用碘克沙醇[OptiPrep]的密度梯度离心法从动物组织和细胞质分离高度纯化的过氧化物酶体所需的一切试剂及详细实验方案。该试剂盒还可用于通过大鼠肝脏、大鼠肾脏、兔肝脏及HEK293和HepG2细胞制备过氧化物酶体。

特點和優勢

  • 专为研究规模应用而配制的提取试剂 - 有助于节省时间,最大限度减少浪费
  • 可获得完整的功能性细胞器 - 所得的过氧化物酶体适于功能性研究、代谢检测、蛋白谱分析和疾病状态分析
  • 兼容结构验证产品 - 可采用随附的细胞色素c还原酶检测试剂盒轻松进行完整性验证(货号:CY0100)

其他說明

收到试剂盒后,蛋白酶抑制剂混合物(产品代码 P 8340)应储存在 –20°C 条件下,OptiPrep 密度梯度培养基(产品代码 O3028)应储存在室温下。

法律資訊

OptiPrep is a trademark of Serumwerk Bernburg AG

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS

象形圖

Corrosion

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


分析證明 (COA)

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C M Rodrigues et al.
Journal of lipid research, 37(3), 540-550 (1996-03-01)
We recently demonstrated that the formation of delta 22-bile acids is a quantitatively major pathway for normal bile acid synthesis in the adult male Sprague-Dawley rat. This pathway is specific for 7 beta-hydroxy bile acids and, when ursodeoxycholic acid is
Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
P B Lazarow et al.
Proceedings of the National Academy of Sciences of the United States of America, 73(6), 2043-2046 (1976-06-01)
Purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-CoA oxidizing system that uses O2 and NAD as electron acceptors. The system was detected by the ability of added palmitoyl-CoA to elicit O2 consumption, H2O2 production, and O2-dependent NAD reduction. The
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
Dieanira Erudaitius et al.
Free radical biology & medicine, 120, 356-367 (2018-03-31)
The high extracellular hydrogen peroxide (H2O2) concentrations generated during pharmacological ascorbate (P-AscH-) therapy has been shown to exhibit a high flux into susceptible cancer cells leading to a decrease in clonogenic survival. It is hypothesized that the intracellular H2O2 concentration

文章

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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