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重要文件

P4020

Sigma-Aldrich

Protamine sulfate

amorphous powder

同義詞:

鲑精朊, 鲑精蛋白 来源于鲑鱼

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77
暫時無法取得訂價和供貨情況

產品名稱

精蛋白 硫酸盐 来源于鲑鱼, Grade X, amorphous powder

種類

Grade X

品質等級

形狀

amorphous powder

雜質

histone, free (Millon test)

顏色

white to off-white

溶解度

6 M HCl: 25 mg/mL

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一般說明

精蛋白是富含半胱氨酸和精氨酸的蛋白。[1]它具有多个磷酸化位点。[2]它位于精子头部。[3]

應用

与由Esterquat-1组成的具有可比性的固体脂质纳米颗粒(SLN)相比,SLN形式的鱼精蛋白可更有效地促进质粒DNA的转染,而且细胞毒性更低。
可用于从蛋白样品中去除DNA或用于纯化DNA结合蛋白。可用作逆转录病毒介导的基因转移中聚凝胺的替代物。

与由Esterquat-1组成的具有可比性的固体脂质纳米颗粒(SLN)相比,SLN形式的鱼精蛋白可更有效地促进质粒DNA的转染,而且细胞毒性更低。
精蛋白硫酸盐(源于鲑鱼)已用于
  • 预处理细胞,研究其对呼吸道合胞病毒感染的作用[4]
  • 慢病毒生产[5]
  • 通过衍生化成为偶氮烯二醇类NO供体[6]

生化/生理作用

硫酸鱼精蛋白是一种可与 DNA 结合并产生沉淀的小阳离子蛋白。它能中和肝素的抗凝血活性,还可抑制脂蛋白脂肪酶。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. Are there other solvents besides 6M HCl to bring protamine sulfate salt into solution?

    1 answer
    1. This product is also soluble in water at 10mg/ml. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/195/258/p4020pis.pdf

      Helpful?

  2. Bonjour, Pourriez vous me confirmer qu'il s'agit bien d'un polypeptide et de combien d'unité de monomère est il formé ? Pourriez vous m'envoyer la représentation de la molécule ? merci

    1 answer
    1. Unfortunately, structural studies on for this product have not been performed. However, regarding structure characterization and molecular representation of protamine from salmon, the following publication may be of interest for reference.

      J Biol Chem. 1959 Sep:234:2314-6.
      Physical and chemical properties of protamine from the sperm of salmon (Oncorhynchus tschawytscha). II. Anion binding characteristics
      https://pubmed.ncbi.nlm.nih.gov/13807973/

      Helpful?

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