推薦產品
應用
磷酸酶抑制剂混合物可通过向能够对细胞提取物中存在的蛋白进行修饰的酶添加抑制剂来提高完整蛋白的产量。 该产品已经在来源于包括人胎盘和牛肝的动物组织以及A431、CHO和U937细胞的提取物上进行了测试。它已经优化并测试用于通过pNPP进行测量的碱性磷酸酶的L-同工酶以及丝氨酸-苏氨酸蛋白磷酸酶。
生化／生理作用
该混合物含有具有特异性抑制属性的独立成分。 斑蝥素可抑制蛋白磷酸酶2A。 (-)-对草酸溴左旋咪唑可抑制碱性磷酸酶的L-同工酶。 花萼海棉诱癌素A可抑制蛋白磷酸酶1和2A。
注意
该产品在供货后可在推荐的2-8°C储存温度下稳定四年。
準備報告
该混合物以溶于二甲基亚砜的透明溶液形式提供。推荐按每500 mg来自组织或细胞提取的蛋白采用1 mL的混合物对其稀释。 通常使用1%（v/v）的终浓度，其可抑制存在于人胎盘和牛肝27,000 × g上清液以及A431、CHO和U937提取物100,000 × g上清液中的磷酸酶活性。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F
閃點（°C）
87 °C
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
磷酸酶抑制劑的使用對於所有類型的磷酸化研究都至關重要。比較磷酸酶抑制劑雞尾酒，選擇最適合您的研究。
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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