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N6655

Sigma-Aldrich

抗-神经生长因子2.5S 兔抗

fractionated antiserum, buffered aqueous solution

同義詞:

抗-NGF-2.5S

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
51111800
NACRES:
NA.41
暫時無法取得訂價和供貨情況

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

fractionated antiserum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse, human

技術

Ouchterlony double diffusion: suitable
western blot: 1:2,000

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... NGF(4803)
mouse ... Ngf(18049)

一般說明

神经生长因子(NGF)属于神经营养蛋白家族。它位于人类染色体上的1p13.2。NGF有两种分子形式,即7S NGF和2.5S NGF,它们对神经元具有相同的神经营养作用。

特異性

可用于监测NGF(2.5S、β和7S)。

免疫原

来自雄性小鼠颌下腺的NGF-2.5S。

應用

成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫荧光(1篇论文)
蛋白质印迹分析(1篇论文)
抗神经生长因子2.5S抗体可用于免疫印迹和斑点印迹,最低抗体工作稀释度为1:2000。使用大鼠中缝大核组织提取物将1:1000的抗体稀释液用于免疫印迹。该抗体用于小鼠星形胶质细胞的免疫荧光和中和测定。

生化/生理作用

该基因的突变导致遗传性感觉和自主神经病变第五类(HSAN-V)。

外觀

磷酸盐缓冲盐水溶液。

免責聲明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Micaela Caserta et al.
Frontiers in neuroscience, 13, 58-58 (2019-02-23)
Previous studies demonstrated exercise-induced modulation of neurotrophins, such as Nerve Growth Factor (NGF) and Brain-Derived Neurotrophic Factor (BDNF). Yet, no study that we are aware of has examined their change as a function of different training paradigms. In addition, the
Liang-Wei Chen et al.
The Journal of comparative neurology, 497(6), 898-909 (2006-06-28)
To address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/Bl mice that had been treated
7S nerve growth factor has different biological activity from 2.5 S nerve growth factor in vitro
Shao N, et al.
Brain Research, 609(1-2), 338-340 (1993)
New protein fold revealed by a 2.3-
McDonald N Q, et al.
Nature, 354(6352), 411-411 (1991)
Anna R Taylor et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(3), 634-644 (2007-01-20)
During development, motoneurons (MNs) undergo a highly stereotyped, temporally and spatially defined period of programmed cell death (PCD), the result of which is the loss of 40-50% of the original neuronal population. Those MNs that survive are thought to reflect

Questions

1–4 of 4 Questions  
  1. Is this antibody N6655 anti-NGF 2.5S against targeting NGFR?

    1 answer
    1. Anti-Nerve Growth Factor 2.5S, catalog number N6655, is produced in rabbits using immunogen 2.5S nerve growth factor purified from mouse submaxillary glands.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. In western blot application, what size protein is recognized by Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit?

    1 answer
    1. Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit, will recognize a 30 kD protein on western blot.

      Helpful?

  4. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

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