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N6009

Sigma-Aldrich

神经生长因子-2.5S 来源于鼠颔下腺

NGF-2.5S, lyophilized powder, suitable for cell culture

同義詞:

NGF-2.5S

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77

生物源

mouse

品質等級

形狀

lyophilized powder

效力

0.1-30 ng/mL EC50 (using PC-12 cells)

分子量

26.5 kDa

包裝

pkg of 4X25 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

mouse ... Ngf(18049)

一般說明

神经生长因子(NGF)由不同类型的细胞生成,例如结构细胞、辅佐细胞和免疫细胞等。它属于神经营养因子家族。

應用

来自小鼠颌下腺的神经生长因子-2.5已用于:
  • Ham′ F-12培养基中,以使用背根神经节(DRG)进行生长锥塌陷测试
  • “工艺室”中进行微流控室研究的培养基补充剂
  • 处理中期黄体细胞,以研究其对胆碱乙酰基转移酶(CHAT)表达的影响

生化/生理作用

神经生长因子(NGF)具有神经营养作用,可维持周围神经系统的交感神经元和感觉神经元以及前脑胆碱能神经元。NGF可促进来自鸡胚背根神经节和大鼠PC12嗜铬细胞瘤细胞的神经突样细丝的生长。NGF可能会在体内胎儿发育和神经再生中发挥作用,尤其在缺血、手术或化学损伤后。NGF也可能与中枢神经系统有关。它对溃疡(人体皮肤、角膜溃疡和压疮)、青光眼、黄斑病变和视网膜色素变性等具有改善作用。NGF-2.5S是一种最初在解离条件下产生的NGF-b,通常因其在纯化过程中会发生蛋白水解而与NGF-b有所不同。

其他說明

神经生长因子-7S(NGF-7S)的2.5S亚基在最初分离的条件下通过Bocchini和Angeletti方法的改进从雄性小鼠颌下腺中分离时基本上是β-亚基。

外觀

从乙酸钠缓冲液中的0.2 μm滤过溶液冻干。

分析報告

采用 3 天 MTT 试验测定生物活性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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