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一般說明
Imprint™ 甲基化 DNA 定量技术提供了一种快速可靠的方法来测量全局 DNA 甲基化移位。印记甲基化 DNA 定量试剂盒包含检测相对水平的甲基化 DNA 所需的所有试剂。最多 200 ng 纯化 DNA 与检测试剂条的微孔结合。使用捕获和检测抗体检测甲基化 DNA，然后用比色法定量。样本中存在的甲基化 DNA 的量与测量的吸光度成正比。
特點和優勢
- 基于 ELISA 的格式
- 在 4 个易于遵循的步骤中完成程序
- 提供的所有试剂，包括对照甲基化 DNA
- 检测限为 5 ng 全甲基化 DNA
- 输入 DNA 可能低至 10-200 ng
- 程序在 3.5 小时内完成
- 96 孔格式允许研究单样本或高通量研究
- 对 5-mC 具有高特异性的优化抗体和试剂；对非甲基化胞嘧啶无交叉反应
- 可与贴壁和悬浮细胞、组织、血浆及其他体液如唾液、尿液和血清一起使用
法律資訊
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
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8A - Combustible corrosive hazardous materials
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文章
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
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Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
染色質免疫共沉淀 qPCR 用於研究不同條件下的基因調控。
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