推薦產品
檢測方法
fluorometric
相關疾病
endocrinological disorders, diabetes; genitourinary disorders; neurological disorders; cancer
儲存溫度
−20°C
一般說明
The Fluo-4 No Wash Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are preloaded with Fluo-4 AM which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals the release of intracellular calcium, which greatly increases the fluorescence of Fluo-4.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
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