推薦產品
檢測方法
colorimetric
相關疾病
gastrointestinal diseases
儲存溫度
−20°C
一般說明
胰蛋白酶（EC 3.4.21.4）是一种胰腺丝氨酸蛋白酶，能够特异性剪切精氨酸和赖氨酸残基羧基侧的肽键。如果剪切位点的任意一侧存在酸性残基，则水解速率降低；如果羧基侧存在脯氨酸残基，则可能不会发生剪切。对目标蛋白质进行胰蛋白酶消化，可产生高度特异性的剪切以及数量有限的肽段。
適合性
适用于检测各种样品中的胰蛋白酶活性。
原則
在该检测中，胰蛋白酶可切割底物以产生 p-硝基苯胺（p-NA），其可在λ= 405nm处进行检测。由于颜色强度与p-NA含量成正比，因此可以对胰蛋白酶活性进行准确测定。该试剂盒可在各种样品中检测10-100mU（p-NA单位）胰蛋白酶。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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條款
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
使用 BAEE 底物的連續分光光度速率測定方法可測量胰蛋白酶的活性，對於酵素的特性分析非常重要。
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