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Merck
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MAK129

Sigma-Aldrich

β-葡萄糖苷酶活性检测试剂盒

sufficient for 100 colorimetric tests

同義詞:

Beta-Glucosidase Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

相關疾病

neurological disorders; gastrointestinal diseases; hematological disorder

儲存溫度

−20°C

一般說明

β-葡糖苷酶可通过作用于末端非还原的b(1→4)-连接的D-葡萄糖残基来水解碳水化合物,同时释放D-葡萄糖。生物体需要β-葡糖苷酶来消化纤维素。溶菌酶,一种存在于泪液中的β-葡萄糖苷酶,可作用于革兰氏阴性细菌的肽聚糖细胞壁中存在的β(1→4)葡萄糖键,并有助于预防眼睛中的细菌感染。β-葡萄糖苷酶活性的缺失与戈谢病′和帕金森病′有关。

應用

β-葡萄糖苷酶活性测定试剂盒已用于测定样品的β-葡糖苷酶活性。

特點和優勢

兼容高通量的操作系统。

適合性

β-葡萄糖苷酶活性检测试剂盒提供了一种对生物样品中的β-葡萄糖苷酶活性进行测定的简单直接的方案,可用于β-葡萄糖苷酶生成的性质分析,以及筛选β-葡萄糖苷酶的调控因子。

原則

在该检测中,β-葡糖苷酶活性通过β-葡糖苷酶水解 硝基苯基-β-D-吡喃葡萄糖苷的反应来确定,该反应可形成比色(405 nm)产物,其与所存在的β-葡糖苷酶活性成比例。一个单位的β-葡糖苷酶表示在pH 7.0条件下,每分钟催化1.0 μmole底物水解所需的酶量。

儲存類別代碼

12 - Non Combustible Liquids


分析證明 (COA)

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