跳轉至內容
Merck
全部照片(3)

重要文件

檢視所有文件

MAK086

Sigma-Aldrich

乙酸盐检测试剂盒（比色法）

sufficient for 100 colorimetric tests

登入查看組織和合約定價
全部照片(3)

About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

儲存溫度

−20°C

一般說明

醋酸是电离形式的乙酸，是脂肪酸和碳水化合物代谢中的关键代谢物。大部分乙酸盐用作辅酶 A（CoA）、乙酰辅酶 A 的缀合物。乙酰辅酶 A 是脂肪酸和胆固醇合成的前体，是柠檬酸循环的重要起始化合物。[1] 乙酰辅酶 A 还用作合成乙酰胆碱的前体和乙酰基的供体，用于组蛋白和非组蛋白蛋白的翻译后乙酰化反应。[2][2]乙酸盐由碳水化合物、氨基酸、脂肪酸和乙醇的代谢产生。

乙酸盐检测试剂盒提供了一种简单直接的方法，用于测量各种样品中的乙酸盐。通过偶联酶测定法测定乙酸盐浓度，其产生与存在的乙酸盐成比例的比色（450nm）产物。

應用

乙酸盐检测试剂盒（比色法）已用于量热测定乙酸盐浓度。[3][4]

適合性

适用于检测血清、血浆、动物组织（肝脏、肾脏、肌肉、心脏等）、细胞培养（粘附或悬浮细胞）和食物中的乙酸盐。

原則

乙酸盐检测试剂盒提供了一种简单直接的方法，用于测量各种样品中的乙酸盐。通过偶联酶测定法测定乙酸盐浓度，其产生与存在的乙酸盐成比例的比色（450 nm）产物。

取代透過

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
8L08K06580
8L11K06580
8B14K06580
8D15K06580
8J27K06580

未看到正確版本？

如果您需要一個特定的版本，您可以透過批號來尋找特定憑證。

搜尋 COA

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Dominic Ritler et al.
Scientific reports, 9(1), 19438-19438 (2019-12-21)
Alveolar echinococcosis (AE) is a zoonotic disease that is deadly if left untreated. AE is caused by the larval metacestode stage of the cestode Echinococcus multilocularis. Better knowledge on the host-parasite interface could yield novel targets for improvement of the
A Pichia anomala Strain (P. anomala M1) Isolated from Traditional Greek Sausage is an Effective Producer of Extracellular Lipolytic Enzyme in Submerged Fermentation.
Papagianni M and Emmanuel M P
Ferroelectr., Lett. Sect., 3(3), 43-43 (2017)
Siegel G J, et al.
Basic Neurochemistry: Molecular, Cellular and Medical Aspects. (1999)
Jeremy D Osko et al.
Biochemistry, 58(36), 3755-3766 (2019-08-23)
Polyamines are small organic cations that are essential for cellular function in all kingdoms of life. Polyamine metabolism is regulated by enzyme-catalyzed acetylation-deacetylation cycles in a fashion similar to the epigenetic regulation of histone function in eukaryotes. Bacterial polyamine deacetylases
Histone H3 T11 phosphorylation by Sch9 and CK2 regulates lifespan by controlling the nutritional stress response.
Workman J L, et al.
bioRxiv, 282384-282384 (2018)
查看所有相關論文

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the minimum detection limit of this assay? What is the quantification range? 

    1 answer

    1. The kit can detect less than 20 μM of Acetate in various samples. The standard curve ranges from 0 - 10 nmole/well.

      Helpful?

  4. What is the enzymatic reaction and the resulting intermediate substance?

    1 answer

    1. Initially, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. This intermediate then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The stated intermediate, considered proprietary, is NADH-based."

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務