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MAK072

Sigma-Aldrich

丙酮酸激酶活性测定试剂盒

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

用途

sufficient for 100 colorimetric or fluorometric tests

檢測方法

colorimetric
fluorometric

相關疾病

hematological disorder; cancer

儲存溫度

−20°C

一般說明

丙酮酸激酶（PK）是催化糖酵解途径中最后一步的酶，能催化将磷酸基团从磷酸烯醇式丙酮酸（PEP）转移到ADP，生成一分子丙酮酸盐和一分子ATP。由于丙酮酸激酶表达或活性的缺陷会造成的丙酮酸激酶缺乏症，是溶血性贫血的第二大常见原因。

丙酮酸酶活性检测试剂盒提供了一种简单且直接的检测多种生物样品中丙酮酸激酶活性的方法。丙酮酸的浓度可通过偶联酶促反应来测定，此反应会生成与样品中的丙酮酸含量成正比的比色(570 nm)/ 荧光(λex = 535/λem = 587 nm)产物。一单位丙酮酸激酶的定义为，在25℃ 条件下每分钟将一个磷酸基团从PEP转移到ADP生成1μmol 丙酮酸所需的酶量。

應用

丙酮酸激酶活性测定试剂盒已用于测定样品中丙酮酸激酶的活性。[1][2][3]

適合性

适用于测量各种生物样本中的丙酮酸激酶活性，如血液、组织和培养细胞等

原則

丙酮酸激酶活性测定试剂盒提供了一种简单直接的方法，来测量各种生物样品中的丙酮酸激酶活性。通过偶联酶测定法测定丙酮酸浓度，其产生与存在的丙酮酸盐成比例的比色（570nm）/荧光（λex = 535/λem = 587 nm）产物。一个单位的丙酮酸激酶是在 25℃ 下，每分钟将磷酸基团从 PEP 转移到 ADP，以产生 1.0 μmole 丙酮酸的酶量。

取代透過

產品號碼
描述
訂價

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334,H412

危險分類

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
8K28K07090
8B03K07090
6K19K07090
6H27K07090
6A04K07090

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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is this assay kit compatible to carry out the assay in Baker's yeast?

    1 answer

    1. This kit may be used with variety of biological samples. However, it has not been tested with yeast samples, hence it cannot be guaranteed as suitable. Suitability would need to be validated by the end user.

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  4. What is the optimal cell quantity for conducting an assay with the Pyruvate Kinase Activity Assay Kit, particularly MAK072, and is this kit appropriate for use with red blood cells?

    1 answer

    1. Optimization for the specific sample type is necessary. Typically, the protocol involves using 1 million cells or 10 mg of tissue suspended in 150-200 microliters of assay buffer.

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  5. 请问一下这个是否适用于红细胞PKR检测，并且是否要破裂细胞，同时有酶标仪和九六孔板即可完成相关检测。

    1 answer

    1. Theoretically, the Pyruvate Kinase (PK) Assay Kit should work with isolated red blood cells (RBC). However, RBC has not been purified or tested with this kit, therefore, no specific protocol recommendations can be made.

      The end-user will have to determine the suitability using a reliable way to isolate RBC. Fresh whole blood coagulates which might negatively influence the isolation of RBC. Hence the isolation of RBC as well as the protocol for the PK Assay kit will have to be optimized for the intended application. This kit is labeled as being able to be used with blood as authors in the following citation used this kit for pyruvate kinase in plasma isolated from whole blood.

      Please see the following publication below which may be beneficial for developing the appropriate method.
      Nguyen Ba Vuong, Ha Van Quang, Bui Ngoc Linh Trang, Dao Hong Duong, Nguyen Linh Toan, Hoang Van Tong,
      Association of PKLR gene copy number, expression levels and enzyme activity with 2,3,7,8-TCDD exposure in individuals exposed to Agent Orange/Dioxin in Vietnam,
      Chemosphere, Volume 329, 2023, 138677, ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2023.138677.

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