SRP0415

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 同義詞: OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
• CAS號碼: 1644286-11-2
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥80% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 59 kDa
• 包裝: pkg of 20 μg
• 製造商／商標名: Sigma-Aldrich
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 1.8 mg/mL
• 技術: cell based assay: suitable
• 溶解度: water: soluble
• NCBI登錄號: NM_182470
• UniProt登錄號: P14618
• 應用: life science and biopharma
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PKM(5315)

描述

一般說明

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2.[1][2] PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues,[2] retina, adipose tissues, and islets.[1] The PKM1 gene is located on the human chromosome at 15q23.[3]

應用

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.[4]
• oxidative stress experiments on PKM2.[5]
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).[6]

生化／生理作用

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.[1]

安全資訊

• 象形圖: GHS08,GHS07
• 訊號詞: Danger
• 危險聲明: H315,H319,H360D
• 防範說明: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危險分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable