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Merck
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重要文件

M7633

Sigma-Aldrich

4-甲基伞形酮-α-D-半乳糖苷

≥98% (TLC)

同義詞:

MU-alpha-GAL

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About This Item

經驗公式(希爾表示法):
C16H18O8
CAS號碼:
分子量::
338.31
Beilstein:
94674
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32
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化驗

≥98% (TLC)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless to very faintly yellow

螢光

λex 317 nm; λem 374 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)

儲存溫度

−20°C

SMILES 字串

CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1

InChI 密鑰

YUDPTGPSBJVHCN-CHUNWDLHSA-N

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應用

4-甲基伞形基-α-D-吡喃半乳糖苷已用作人工底物,测定 α-半乳糖苷酶-A 活性。[1][2][3]

生化/生理作用

4-甲基伞形基α-D-吡喃半乳糖苷是 α-半乳糖苷酶-A 的荧光底物。[4]这种酶将底物转化为蓝色荧光产物甲基伞形花。[3][4]通过分光光度法测定荧光强度。[4]

準備報告

4-甲基伞形酮α-D-吡喃半乳糖苷可溶于水(50 mg/ml),形成无色至淡黄色的澄清溶液(加热处理后)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
Zhen-Dan Shi et al.
Analytical and bioanalytical chemistry, 394(7), 1903-1909 (2009-06-13)
Alpha-galactosidase A hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins in lysosomes. Mutations in alpha-galactosidase cause lysosomal accumulation of the glycosphingolipid, globotriaosylceramide, which leads to Fabry disease. Small-molecule chaperones that bind to mutant enzyme proteins and correct their misfolding
Eugènia Ruiz-Cánovas et al.
Colloids and surfaces. B, Biointerfaces, 208, 112123-112123 (2021-09-28)
The capability of HeLa cells to internalize large spherical microparticles has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8 µm of diameter. In both absence but especially under the action of a magnet, both types of particles were
Tali Kizhner et al.
Molecular genetics and metabolism, 114(2), 259-267 (2014-08-27)
Fabry disease is an X-linked recessive disorder caused by the loss of function of the lysosomal enzyme α-Galactosidase-A. Although two enzyme replacement therapies (ERTs) are commercially available, they may not effectively reverse some of the Fabry pathology. PRX-102 is a
Ronald K Gary et al.
Analytical biochemistry, 343(2), 329-334 (2005-07-12)
Senescence-associated beta-galactosidase activity is a widely used biomarker for assessing replicative senescence in mammalian cells. This enzymatic activity has generally been measured by staining cells with the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) at pH 6.0, a reaction condition that suppresses lysosomal

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